Δ<i>wsc-1</i>, Δ<i>ham-7</i>, and Δ<i>wsc-1;</i>

Abstract

<p><b>Δ</b><b><i>ham-7</i></b><b> are deficient in MAK-1 activation.</b> (<b>A</b>) In the upper panel, extracts of non-stressed and oxidatively stressed wild type, Δ<i>wsc-1</i>, Δ<i>ham-7</i>, and Δ<i>wsc-1;</i> Δ<i>ham-7</i> cells were prepared and assayed for the presence of phosphorylated MAK-1 and MAK-2 by a Western blot assay using antibody that specifically recognizes the phosphorylated proteins. Extracts from non-stressed and from stressed cells are denoted by – and +. The sizes of the MAK-1 and MAK-2 proteins are shown at the side of the Western blot. A Western blot against tubulin was used as a control and to calibrate the amounts of protein in each of the samples. The amounts of activated MAK-1 (middle panel) and MAK-2 (lower panel) in each of the samples relative to the amount of activated MAK-1 or MAK-2 in the non-stressed wild type cell are shown (n = 5). (<b>B</b>) The colony extension rates for wild type, Δ<i>wsc-1</i>, Δ<i>ham-7</i>, Δ<i>wsc-1;</i> Δ<i>ham-7</i>, and Δ<i>mak-1</i> are shown. (<b>C</b>) The colony morphology (upper panel), the lack of protoperithecia production (middle panel), and lack of CAT fusion (lower panel) are shown for Δ<i>wsc-1;</i> Δ<i>ham-7</i>, and Δ<i>mak-1</i>.</p