Alignment of the ACTB mRNA with six PGs that are the most homologous to the ACTB mRNA shows that the ACTB mRNA (after deletion of the poly-A tail) has no unique region that is long enough to be a primer.

Abstract

<p>The 1452–1473rd nt and the 1678–1697th nt regions of the ACTB mRNA (underlined) encompass the most mismatches compared with other regions, and thus are selected as the forward and reverse primers, respectively.</p