Alignment of the Actb mRNA with the six PGs that are the most homologous to the Actb mRNA shows that the Actb mRNA has no unique region that is long enough to be a primer.

Abstract

<p>The 1471–1489th nt and 1852–1870th nt regions of the Actb mRNA (underlined) have the most mismatches compared with other regions and thus are used as the forward and reverse primers, respectively.</p