Indirect calorimetry.

Abstract

<p>At 6 weeks after the initiation of the experiment, oxygen consumption (<i>V</i>O₂) and carbon dioxide production were recorded every 2 min using indirect calorimetry. The measurements were performed following the diet-switch protocol in individual mice (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043764#pone-0043764-g001" target="_blank">Fig. 1B</a>). During the first part of the measurements (between 6.00 p.m. and 8.00 a.m.), animals had <i>ad libitum</i> access to water and various cHF-based diets. After that period, the animals were fasted for 10 hours. At the beginning of the dark cycle at 6.00 p.m., all subgroups were switched to Chow diet, and the measurements continued for 20 more hours (‘Re-feeding Chow ‘). The measurements were performed under the 12-hour light-dark cycle (lights on from 6∶00 a.m.) at ambient temperature of 22°C. Data are means±SE (<i>n</i> = 5; mice randomly chosen from each subgroup, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043764#pone-0043764-t001" target="_blank">Table 1</a>) expressed for the following three time-periods (i) from 0.00 p.m. to 8.00 a.m., feeding various cHF-based diets; (ii) from 9.00 a.m. to 5 p.m., fasting; and (iii) from 0.00 p.m. to 8.00 a.m., re-feeding Chow. ΔRER, the difference in RER between mice re-fed Chow diet and fasted mice.</p>a<p>Significantly different from cHF diet;</p>b<p>significantly different from cHF+ROSI diet (ANOVA).</p