Phospho-proteomic profiling of E<sub>2</sub>-BSA and puerarin effects on ERK1/2 phosphorylation in ESCs and confirmed by western blot analysis.
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Abstract
<p>Cells were treated as indicated. A. AKT, Chk2 and ERK1/2 were activated by E<sub>2</sub>-BSA and suppressed by puerarin. B. Western blot analysis of E<sub>2</sub>-BSA on phosphorylation in ESCs. Cells were treated with vehicle (DMSO) or E<sub>2</sub>-BSA (10<sup>−8</sup> mol/L) for 15 min. AKT, Chk2 and ERK1/2 were activated by E<sub>2</sub>-BSA. Expression was normalized to total ERK1/2.</p
