Effect of E₂-BSA and puerarin on cell proliferation in ESCs.

Abstract

<p>(A) ESCs were treated with various concentrations (10⁻¹⁰ to 10⁻⁶ mol/L) of E₂-BSA for 4 d. (B) Growth curves of ESCs incubated with E₂-BSA (10⁻⁸ mol/L) and/or puerarin (10⁻⁹ mol/L) for 0, 1, 2, 4 d. (C) Cells were treated for 4 d with E₂-BSA (10⁻⁸ mol/L) in the absence or presence of puerarin (10⁻⁹ mol/L) and/or pretreated with U0126 (20 µmol/L) for 60 min. The figures represent data obtained from three experiments; each experiment was performed in triplicate, and data are represented as the mean ± SD. DMSO treatment was used as the vehicle control. <i>Vs. control</i>,**, <i>P</i><<i>0.01,</i> *, <i>P</i><<i>0.05; vs. E₂-BSA, ▴, P</i><<i>0.05.</i></p