Copy numbers of OSBP/ORP mRNAs in human adipose tissues and SGBS adipocytes.
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Abstract
<p>The mRNAs were quantified by qPCR using the corresponding cDNAs as calibrators (see Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045352#s2" target="_blank">Methods</a>), and the mRNA quantities are presented on a log10 scale as copies/ng total RNA. A. Subcutaneous adipose tissue; B. Visceral adipose tissue. The data represents mean ± S.E., n = 4. C. Simpson-Golabi-Behmel syndrome (SGBS) adipocytes after 22-day differentiation. The mean from a single experiment carried out in triplicate is shown.</p