Intracellular production of pro-inflammatory cytokines and chemokines in monocytes upon engagement of Siglec-7.

Abstract

<p>(A) Representative flow cytometry dot plot graphs showing the percentage of CD14^pos monocytes, within total PBMCs, producing IL-6, IL-1α, CCL4/MIP-1β, IL-8 and TNF-α after incubation with either the anti-Siglec-7 mAb (lower line) or the matched IgG2b isotype control (upper line). (B) Statistical summary graphs of dot plots with medians (horizontal black bars) and p values showing the percentage of CD14^pos monocytes producing IL-6, IL-1α, MIP-1β, IL-8 and TNF-α in response to either the anti-Siglec-7 mAb (red circles) or the matched IgG2b isotype control (green circles).</p