Detection of cytokines in PBMC supernatant upon engagement of Siglec-7.

Abstract

<p>Secretion of IL-6, IL-1α, IL-8, CCL4/MIP-1β, GRO and DAN in the supernatant of PBMCs stimulated with the anti-Siglec-7 mAb (right panel) compared with that of PBMCs incubated with a matched IgG2b isotype control (left panel). The culture medium was collected and analysed using a semi-quantitative protein array detecting simultaneously 507 human soluble proteins. Data shown in this figure are representative of 3 independent experiments.</p