Zymosan-induced production of TNFα and IL-1α: silencing of Siglec-7.

Abstract

<p>(A) Representative graphs showing the intracellular production of TNF-α and IL-1α in monocytes transfected either with non-targeting SiRNA control probes (green line in the histogram graph and dot plot graphs in the upper line of the panel) or with SiRNA duplexes specific for Siglec-7 (red line in the histogram graph and dot plot graphs in the lower line of the panel) in response to <i>Zymosan</i>. MFI: Mean Fluoresence Intensity. Filled gray Histogram: isotype control. (B) Summary graphs of dot plots (left) and statistical summary bar graphs with p values and standard deviation (right) showing the percentages of decrement of TNF-α (left) and IL-1α (right) in monocytes silenced for Siglec-7 compared to monocytes not silenced for Siglec-7 in response to <i>Zymosan</i>. Data are representative of 5 independent experiments (± SD).</p