Cortisol effect on liver plasma membrane topography and surface adhesion.

Abstract

<p>A) Representative atomic force microscopy (AFM) images of supported hepatic plasma membrane topography (membrane height). Images were taken prior to (a) and 30 min after cortisol (100 ng/mL) treatment (e) in liquid cell at room temperature. A zoomed in scan is also shown for the control (c) and cortisol treated (g) membranes that was scanned for 60 min. The approximate scan region of the zoomed in image is indicated by the dashed red box in the control image (a) and solid red box in the cortisol-treated image (e). Two distinct domains, which differ in height, are visible in both control and cortisol-treated membranes. A representative higher domain is indicated by the dotted arrow, while the lower domain is indicated by the solid arrow (c). Short-term cortisol treatment altered the topography of the plasma membrane. The cross-section graph featured below each image was calculated from points along the white horizontal line. The y-axis represents vertical height (nm), whereas the x-axis represents the horizontal distance (nm). B) Representative AFM images of supported hepatic plasma membrane phase (surface adhesion properties). Images were taken prior to (a) and 30 min after cortisol (100 ng/mL) treatment (e) in liquid cell at room temperature. A zoomed in scan is also shown of the control (c) and cortisol treated (g) membranes that was scanned for 60 min. The approximate scan region of the zoomed in image is indicated by the dashed red box in the control image (a) and solid red box in the cortisol-treated image (e). Two distinct domains, which differ in their viscoelastic (surface adhesion) are visible in both control and cortisol-treated membranes. Acute cortisol treatment altered the viscoelastic properties of the plasma membrane within 30 min of treatment. The cross-section graph featured below each image was calculated from points along the white horizontal line. The y-axis represents degree of deflection (degrees), whereas the x-axis represents the horizontal distance (nm). C) A schematic representation of cortisol’s effect on plasma membrane properties. Short-term incubation with cortisol (b) increased surface roughness (height difference between higher and lower domains) compared to control membrane (a).</p

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