<p>(<b>A</b>) Immunofluorescence microscopic images of sagittal sections of developmental mouse retina tissue array stained with anti-H3K4me2 (<b>A</b>, green, upper panels) and anti-Rhodopsin (<b>A</b>, red, upper panels), anti-H3K27me3 (<b>A</b>, green, lower panels) and anti-SVP38 (<b>A</b>, red, lower panels), and counterstained with Hoechst in cell nuclei (<b>A,</b> blue). ONBL, outer neuroblast layer; INBL, inner neuroblast layer; GCL, ganglion cell layer; ONL, outer nuclear layer; INL, inner nuclear layer. Scale bar represents 25 µm. (<b>B</b>) H3K4me2 labeling with high magnification for ONL from adult retina (Scale bar = 6 µm). (<b>C</b>) H3K27me3 labeling with high magnification for ONL from adult retina (Scale bar = 6 µm). (<b>D</b>) Averaged and normalized intensity profiles for fluorescence of each specific antibody or Hoechst staining across the nuclear centers (e.g. white bars in <b>B</b> and <b>C</b>) of rod photoreceptor nuclei (n = 5). (<b>E</b>) Adult retina outer nuclear layer labeled with an antibody recognizing Crx. (<b>F</b>) Control labeling of adult ONL showing lack of staining with secondary antibody alone. (<b>G</b>) Averaged and normalized intensity profile for Crx labeling (green) and Hoechst (blue) across rod nuclear centers (bar in <b>E</b>). (<b>H, I</b>) Confocal images with high magnification for RGCL from adult retina (Scale bar = 15 µm). Cells marked * or # show distinct cellular distribution with H3K4me2 antibody (<b>H</b>, green) and a′ or b′ show distinct cellular distribution with H3K27me3 antibody (<b>I</b>, green).</p
