NOCE regulates endogenous allele-specific <i>Noto</i> expression.

Abstract

<p>(A) Schematic view of the targeting strategy to delete NOCE in ES cells carrying alleles with green or red fluorescent proteins (GFP and RFP) in the <i>Noto</i> locus, respectively. (B) fluorescence of GFP and RFP in chimeric embryos with the parental heteroallelic cell line (B a, d), and cells in which NOCE was deleted from either the GFP (B b, e) or RFP allele (B c, f). (C) Fluorescence intensities of GFP and RFP and ratio of GFP/RFP of control chimeras, and of targeted/non-targeted (reference) alleles for chimeric embryos with one deleted NOCE allele (see also material and methods and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047785#pone.0047785.s001" target="_blank">Figure S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047785#pone.0047785.s010" target="_blank">Table S3</a>).</p

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