Rabip4’ and AP-3 colocalize on endosomes.

Abstract

<p>HeLa cells were labeled with antibodies against δ-adaptin (<b>A</b>) or δ-adaptin (red) and endogenous rabip4s (green) (<b>B</b>). Scale bar is 10 µm (<b>A</b>) and 2.5 µm (<b>B</b>). HeLa cells expressing VSVG-rabip4’ were labeled with a rabbit antibody against rabip4’ (green) and monoclonal antibodies against the δ subunit of AP-3, the γ1 subunit of AP-1, and the α subunit of AP-2 (all in red). Scale bar is 10 µm. (<b>C</b>). Arrows in insets indicate structures on which AP-3 and rabip4s or VSVG-rabip4’ colocalize (<b>B and C</b>). Extent of colocalization between rabip4’ and the adaptor complexes (<b>D</b>).</p