Interacting domains.

Abstract

<p>Domain organization of rabip4’ and β3-adaptin (<b>A</b>). The binding domain of AP-3 on rabip4’ was determined with overlapping truncations of rabip4’ in a GST pull-down assay using detergent extracts from rescued <i>mocha</i> fibroblasts. Bound AP-3 was analyzed by Western blotting with antibodies against δ-adaptin. The AP-3 binding site was contained within the FYVE domain of rabip4’ (<b>B</b>) and is specific for rabip4’ since the FYVE domains of Hrs and EEA1 did not bind AP-3 (<b>C</b>). A pull-down assay with GST-rabip4’ and ³⁵S-labeled Myc-tagged β3A truncations showed that the rabip4’ binding site is in the hinge region of β3A (<b>D</b>).</p