Rabip4’, EEA1, and Hrs partially colocalize on endosomes.

Abstract

<p>HeLa cells were labeled for endogenous rabip4s and EEA1. Arrows denote co-distribution of the two FYVE domain-proteins. Bottom row represents a blow up of indicated areas. The dashed line marks the contour of cells. Scale bar is 10 µm (<b>A</b>). Ultrathin cryosections of HeLa cells expressing VSVG-rabip4’ were immunogold labeled with polyclonal anti-VSVG (10 nm gold particles). VSVG-rabip4’ localized to the endosomal vacuole (E), as well as to surrounding tubular-vesicular membrane profiles characteristic for recycling tubules (arrows). P is plasma membrane. Bar, 200 nm (<b>B</b>). HeLa cells transfected with GFP-rabip4’ and HA-Hrs were labeled for EEA1 (blue) and HA-Hrs (red), while rabip4’ was visualized by EGFP fluorescence. Bottom row represents an enlargement of the indicated areas. Arrows point to endosomes that contain rabip4’, EEA1, and Hrs, and arrowheads indicate endosomes devoid of rabip4’. Scale bar is 10 µm (<b>C</b>).</p