Transcription was affected by 50 µM Curcumin treatment.

Abstract

<p>(A). Quantitative RT-PCR results of mRNA levels of tested genes. Spermatids were incubated with 50 µM Curcumin for 3 h before qPCR executed (Mean ± SD, n = 3). #<i>p<</i>0.05. (B). Quantitative RT-PCR results of mRNA levels of tested genes. Spermatids were incubated with 50 µM Curcumin for 48 h before qPCR executed (Mean ± SD, n = 3). #<i>p<</i>0.05. *<i>p<</i>0.01. (C). Global transcription status illustrated by <i>in vitro</i> run-on assay. Step: Developmental steps of spermiogenesis. Red: Signals of BrUTP incorporation. Green: Acrosomes highlighted with lectin PNA. Blue: Nuclei counterstained by Hoechst 33342. Bars = 5 µm.</p