A20-silenced Mф immunization enhances NK cell-, CD8⁺ T cell- and CD4⁺ T cell-mediated cytotoxicity.

Abstract

<p>Splenocytes pooled from 2–3 immunized mice were cultured overnight for NK-mediated cytotoxicity assay or 5–6 days in the presence of OT-I or OT-II peptide for T cells-mediated cytotoxicity assay. The splenocytes cultured with OT-II peptide were selected using anti-CD4 beads prior to cytotoxicity assay. Cytotoxic activities were analyzed by LDH release assay as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048930#s4" target="_blank">Material and Methods</a>. Experiments were repeated three times with similar results. *p<0.05, Ad-shA20-Mф immunization vs. Ad-con-Mф immunization for specific killing.</p