Representative patterns of chromatin associated factors expression in spermatids treated with 50 µM Curcumin for 48

Abstract

<p> <b>h.</b> (A). Immunostaining of TBP, TAF1, AP2α, TOPOIIβ, H3K4Me3 and H4K20Me3 in Curcumin-treated spermatids. Red: Signals of given target. Green: Acrosomes highlighted with lectin PNA. Blue: Nuclei counterstained by Hoechst 33342. Bars = 5 µm. (B). Immunostaining of Hdac1 in Curcumin-treated spermatids. Red: Signals of given target. Green: Acrosomes highlighted with lectin PNA. Blue: Nuclei counterstained by Hoechst 33342. Bars = 5 µm. The quantitative analysis of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048673#pone-0048673-g006" target="_blank">Figure 6</a> was listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048673#pone.0048673.s005" target="_blank">Table S3</a>.</p