A20-silenced Mф enhances expression of granzyme B in CD4⁺ T cells, CD8⁺T cells or NK cells.

Abstract

<p><b>A,</b> adenoviral-transduced BMMфs were cocultured with freshly isolated OT-I (<b>uppe</b>r) or OT-II cells (<b>lower</b>) at a raito of 1∶10. 3–5 days later, the cocultured T cells were harvested for analyzing expression of granzyme B by ICS. The data is shown as a representative of 3 independent experiments. (p<0.05, OT-I/shA20-Mф vs. OT-I/con- Mф; p<0.01, OT-II/shA20-Mф vs. OT-II/con-Mф). <b>B,</b> C57BL/6 mice (5–6 mice/group) were immunized (<i>i.p</i>) twice with different adenoviral-transduced Mфs or PBS. Lymphocytes were isolated from the inguinal LNs to analyze expression of granzyme B in NK cells, CD8<b>⁺</b> or CD4<b>⁺</b> T cells by ICS. <b>C.</b> C57BL/6 mice were immunized (<i>i.p</i>) twice with OT-II-pulsed, different adenoviral-transduced BMMфs or PBS. Splenocytes were harvested and in vitro restimulated with OT-II peptide for 48 hrs. CD4⁺ T cells were isolated for analysis of granzyme B expression by qPCR. The data is shown as a representation of three independent experiments. (* p<0.01, shA20- Mф-mice vs. con- Mф-mice).</p