A20-silenced Mф elicits a cytotoxic CD4⁺ T cell response via activation of IFN-γ signaling and by an MHC-class-II-restricted mechanism.

Abstract

<p>A. Adenoviral-transduced BMMфs were used to immunize IFNGR^−/− mice or the wildtype littermates (2–3 mice/group) twice. The inguinal LNs were harvested for analyzing expression of granzyme B in CD4⁺ or CD8⁺ T cells by ICS. p<0.01 Ad-shA20-IFNGR KO mice vs. Ad-ShA20 WT mice. B. Adenoviral-transduced BMMфs were used to immunize Stat1^−/− mice or the wild-type littermates twice (2–3 mice/group). The LNs were harvested for analyzing expression of granzyme B in CD4⁺ (p<0.05, Ad-shA20-Stat1 KO mice vs. Ad-shA20-WT mice) or CD8⁺ T cells by ICS. C. BMMфs were prepared from MHCII^−/− mice or the wild-type littermates. The adenoviral-transduced BMMфs were used to immunize wild-type mice (2–3 mice/group) twice. The LNs were harvested for analyzing expression of granzyme B in CD4⁺ (p<0.01, Ad-shA20-MHC-II KO Mф immunization vs. Ad-shA20-WT Mф immunization) or CD8⁺ T cells by ICS. Experiments were repeated with similar results.</p