Ectopic ALKBH4 expression does not change H3K79 methylation levels <i>in vivo</i>.
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Abstract
<p>As analyzed by Western blotting, all three methylation states (mono- di- and tri-methylation) of the H3K79 residue remained similar in histones purified from stable HEK293 transfectants after doxycycline-dependent over-expression of either ALKBH4 or an enzymatically inactive mutant (ALKBH4<sup>H169A/D171A</sup>), compared to the equivalent, non-induced cells. Signal intensities of bands corresponding to methylated histones were quantified using ImageJ <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049045#pone.0049045-Schneider1" target="_blank">[69]</a>, and normalized to the total histone H3 load, but no effect of ALKBH4 overexpression was detected (not shown).</p
