Analysis of transcript levels of <i>TrRas1</i> and <i>TrRas2</i> mRNA by qRT-PCR.

Abstract

<p>(A) Transcript abundances of <i>TrRas1</i> and <i>TrRas2</i> on different carbon sources. The ratios of the expression of <i>TrRas1</i> and <i>TrRas2</i> to that of the actin reference gene were calculated in <i>T. reesei</i> QM9414 cultured on glycerol, glucose or cellulose. The relative mRNA levels were presented by setting the amount of <i>TrRas2</i> mRNA detected on glucose at 6 h as 1. (B) Quantitative real-time PCR analysis of <i>TrRas1</i> or <i>TrRas2</i> mRNA levels in the Δ<i>TrRas2</i> or Δ<i>TrRas1</i> mutant. The ratios of the expression of <i>TrRas1</i> and <i>TrRas2</i> to that of the actin reference gene were calculated. The relative mRNA levels were presented by setting the amount of <i>TrRas2</i> mRNA in <i>T. reesei</i> TU-6 detected at 12 h as 1. Values are means of three independent experiments. Error bars represent standard deviations.</p