TrRas2 influences cellulase formation in <i>T. reesei</i>.

Abstract

<p>Comparison of Cellobiohydrolase activity (A) and extracellular protein concentration (B) of stains TU-6-Z, Δ<i>TrRas2</i> and <i>PAnigpdA</i>-<i>TrRas2^G16V</i> grown on 1% Avicel cellulose. (C) SDS-PAGE of secreted proteins in culture supernatants from the TU-6-Z, Δ<i>TrRas2</i> and <i>PAnigpdA</i>-<i>TrRas2^G16V</i> strains upon growth on 1% Avicel cellulose. Transcript levels of the major cellulase genes <i>cbh1</i> (E) and <i>cbh2</i> (F) were detected just before inducing (BF, 0 h) and 12 h, 20 h, 28 h, 36 h, 44 h after the beginning of the cultivation on cellulose and 10 h, 20 h on glucose. The relative <i>cbh1</i> and <i>cbh2</i> mRNA levels were present by setting the amount of <i>cbh1</i> and <i>cbh2</i> mRNA obtained from BF as 1 respectively. Each reaction was done in triplicate.</p