TrRas1 and TrRas2 play similar roles in increasing cAMP content.

Abstract

<p>(A) The cAMP levels of TU-6 and Δ<i>TrRas1</i> on PDA plates. (B) Comparison of the cAMP levels of strains TU-6-Z, Δ<i>TrRas2</i> and <i>PAnigpdA</i>-<i>TrRas2^G16V</i>. Cultivation of respective strains and cAMP level determination were performed as described in the methods. The cAMP content is given in relation to parental strain.(C) Phenotypes of wild-type and Δ<i>TrRas1</i> upon growth on PDA plates in the presence or absence of 2 mM cAMP in the medium. Strains were grown for 7 days at 30°C. Scale bars are shown in the figure.</p