Inhibition of miR-200c in BT474 cells causes chemoresistance to doxorubicin treatment.

Abstract

<p>A) miR-200c expression after inhibiton. 24 hours after transfection with either miR-200c inhibitor or scrambled control, BT474 cells were harvested for RNA isolation and quantitative RT-PCR. miR-200c expression was normalized to miR-191 and presented as ratio. B) Cell morphology. Micrographs (phase contrast) of BT474 cells were taken 24 hours after transfection with either miR-200c inhibitor or scrambled control. C) Susceptibility to doxorubicin treatment. BT474 and MDA-MB 436 cells, transfected with inhibitor or scrambled control, were treated with 1, 10 and 20 µM doxorubicin for 72 hours. Cell viability was analyzed using CellTiter Glo. Experiments were done in triplicates with at least two biological replicates. For statistical analysis a student’s t-test was performed. (ns = not significant; *p<0.05; **p<0.01; ***p<0.001).</p