Overexpression of miR-200c in MDA-MB 436 cells increases susceptibility to doxorubicin.

Abstract

<p>A) miR-200c levels after overexpression. After three consecutive transfections with either pre-miR-200c or scrambled control, MDA-MB 436 cells were harvested for RNA isolation and quantitative RT-PCR. miR-200c expression was normalized to miR-191 and presented as ratio. B) Cell morphology. Micrographs (phase contrast) of MDA-MB 436 cells were taken after three consecutive transfections with either pre-miR-200c or scrambled control. C) Susceptibility to doxorubicin treatment. With pre-miR-200c or scrambled control transfected MDA-MB 436 and BT474 cells were treated with 1, 5 and 10 µM doxorubicin for 72 hours. Cell viability was determined by a CellTiter Glo assay. Experiments were done in triplicates with at least two biological replicates. For statistical analysis a student’s t-test was performed. (ns = not significant; *p<0.05; **p<0.01; ***p<0.001).</p