The protein expression of TrkB and Bmi1 is regulated during the rounds of the Molecular Evolution Assay in BT474 cells.

Abstract

<p>Total cell lysates of BT474 cells derived from R0, R2 and R4 of the Molecular Evolution Assay were subjected to western blot analysis to determine protein expression of A) TrkB isoforms, B) p-Akt, C) Bmi1 and D) p53. Actin was used as loading control.</p