IL-1β signaling and the NRLP3 inflammasome are required for immunity against WNV.

Abstract

<p>6–10 wk old age matched WT (closed circles; n = 26) or <i>Il-1r^−/−</i> (open squares; n = 14) (<b>A</b>) or <i>Caspase-1^−/−</i> (open circles; n = 12) and <i>Nlrp3^−/−</i> (closed square; n = 12) (<b>D</b>) or WT (closed circles, n = 5) and <i>Nlrc4^−/−</i> (open circles; n = 4) (<b>E</b>) or WT (closed circles, n = 5) and <i>Myd88^−/−</i> (closed square; n = 5) (<b>F</b>) animals were infected with 100 PFU WNV-TX via s.c. foot-pad inoculation and monitored for survival over the course of 14–16 days (<b>A, D–F</b>). Infected WT (closed circles) or <i>Il-1r^−/−</i> (open squares) animals from panel A, were monitored daily for weight loss (<b>B</b>) or scored for hind limb paralysis and morbidity (<b>C</b>) to day 16 post infection. *p<0.05, ** p<0.005, *** p<0.0005.</p