Effect of the presence of the <i>ACVR1/Alk-2</i> 3′UTR sequence on reporter gene expression.

Abstract

<p>A) The whole <i>ACVR1/Alk-2</i> 3′UTR sequence and both the proximal and the distal derived fragments, carrying the miR binding sites containing module (3′UTR M) and the ARE sequences containing segment (3′UTR A), respectively, were subcloned into the pGL3 Promoter vector downstream the Luciferase coding sequence. B) After transient transfection in the indicated cell lines, reporter activity was measured and normalized against the pRL-SV40 expressing the Renilla Luciferase gene for transfection efficiency. Reporter gene activity of the construct carrying the <i>ACVR1-Alk2</i> 3′UTR (dark grey bars), or the M (pGL3+3′UTR M), and the A (pGL3+3′UTR A) fragments are reported as percentage of the activity obtained with the vector lacking the 3′UTR sequence (pGL3-PV, black bars) considered as 100%. Results are from at least two independent transfection experiments made in triplicate (RLU, Relative Luciferase Units). Two-tailed <i>Student</i>’<i>s t-test</i> was performed and significant differences in comparison to the activity of the pGL3-PV empty vector are given as: <i>p</i><0.05*; <i>p</i><0.01**; or <i>p</i><0.001***.</p

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