Effect of selected miRs on the expression of the <i>ACVR1/Alk-2</i> 3′UTR reporter construct.

Abstract

<p> Relative Luciferase activity obtained after co-transfection of the <i>ACVR1/Alk-2</i> 3′UTR reporter construct with either a scrambled mir Cy3-conjugated as negative control (Black histograms), or each of the indicated miR (light grey), mir148b (A), mir365 (B) and mir26a (C) or with the corresponding anti-miR (dark grey histograms). The reporter gene activity measured in presence of the Cy3-miR negative control is considered as 100 for each independent experiment, RLU, Relative Luciferase Units. The statistical significance of the effect of miR compared to that obtained with the non targeting negative control was evaluated by <i>Student’s T-test</i> analysis, with <i>p</i><0.05*; <i>p</i><0.01**; or <i>p</i><0.001***.</p