<i>ACVR1/Alk-2</i> mRNA is well conserved among species and is unstable in presence of ActD.
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Abstract
<p>A) Output of the comparative genomic analysis obtained with a dedicated software available at the Vista Genome Browser (<a href="http://genome.lbl.gov/vista/index.shtml" target="_blank">http://genome.lbl.gov/vista/index.shtml</a>, tools for comparative analysis*). Non coding Conserved sequences corresponding to the <i>ACVR1/Alk-2</i> 3′UTR are shown as light blue peaks, and are shown relative to their position on human chr2 (March 2006, release) compared to the mouse (first track) rat (second track) and chicken (third track) genomes, respectively, the percentage of identity is indicated on the vertical axis. (*Frazer KA, Pachter L, Poliakov A, Rubin EM, Dubchak I. VISTA: computational tools for comparative genomics. Nucleic Acids Res. 2004 Jul 1;32 (Web Server issue):W273-9). B) and C) C2C12 cells were treated with the ActD transcription inhibitor with the doses and for the time points indicated. <i>ACVR1/Alk-2</i> mRNA was measured by semi-quantitative (B) and quantitative RT-PCR (RT-qPCR, <b>C</b>). Two-tailed student′s t-test was performed. Significant differences in comparison to untreated cells are given as: <i>p</i><0.05*; <i>p</i><0.01**; or <i>p</i><0.001***.</p