Comparison of binding affinity of GP<sub>1,2</sub>-immunized versus sGP-immunized antisera for sGP and GP<sub>1,2</sub>.
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Abstract
<p>(A) Determining apparent K<sub>d</sub> value of antibodies from immunized mice for GP<sub>1,2</sub> and sGP. Antiserum from five mice immunized against GP<sub>1,2</sub> and five mice immunized against sGP were individually analyzed by quantitative ELISA using GP<sub>1,2</sub> (blue) or sGP (red) as coating antigen. Scatchard analysis was used to calculate apparent dissociation constants (K<sub>d</sub>). (B) Comparison of antibody affinity for GP<sub>1,2</sub> and sGP. Comparison of apparent K<sub>d</sub>'s of GP<sub>1,2</sub>-immunized and sGP-immunized polyclonal antisera for sGP (red) and GP<sub>1,2</sub>(blue) was determined by nonlinear regression analysis of Scatchard plots. K<sub>d</sub>'s for sGP and GP<sub>1,2</sub> were calculated for five individual mice in each group and values for the same animal are connected by a black line.</p
