Protective efficacy of immunization with NM2e formulated with Al(OH)₃ and CpG in mice.

Abstract

<p>Groups of 15 mice were immunized with NM2e protein or NM2e formulated with adjuvant and were challenged with 20 LD₅₀ of influenza virus PR8. Mice immunized with normal saline or adjuvant alone were challenged as negative controls. Mice were monitored daily for 21 days after PR8 challenge. Mice were weighed daily to detect morbidity (A). Average weights in each treatment group were followed for the duration of the study, and the percentage of the original body weight was calculated based on the average starting weight for each group at day 0. Survival rates (B) following the challenge within each experimental group were calculated. Tables above the graph compare the results for groups 3, 4, 5, and 6. *, <i>p</i>≤0.05; **, <i>p</i>≤0.01; ***, <i>p</i>≤0.001; ns, not significant.</p