Novel Xylanase from a
Holstein Cattle Rumen Metagenomic
Library and Its Application in Xylooligosaccharide and Ferulic Acid
Production from Wheat Straw
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Abstract
A novel gene fragment containing a xylanase was identified
from
a Holstein cattle rumen metagenomic library. The novel xylanase (Xyln-SH1)
belonged to the glycoside hydrolase family 10 (GH10) and exhibited
a maximum of 44% identity to the glycoside hydrolase from Clostridium thermocellum ATCC 27405. Xyln-SH1 was
heterologously expressed, purified, and characterized. A high level
of activity was obtained under the optimum conditions of pH 6.5 and
40 °C. A substrate utilization study indicated that Xyln-SH1
was cellulase-free and strictly specific to xylan from softwood. The
synergistic effects of Xyln-SH1 and feruloyl esterase (FAE-SH1) were
observed for the release of xylooligosaccharides (XOS) and ferulic
acid (FA) from wheat straw. In addition, a high dose of Xyln-SH1 alone
was observed to improve the release of FA from wheat straw. These
features suggest that this enzyme has substantial potential to improve
biomass degradation and industrial applications