ESI-MS MS/MS MRM and HPLC conditions for each sphingolipid.

Abstract

<p>Seg, segment; RT, retention time; Pre-ion, precursor ion; Pro-ion, product ion; CE, collision energy; ISTD, internal standard.</p>*<p>Cer(d18∶1/26∶1) and Cer(d18∶1/26∶0) were quantitated using the calibration curve of the closest counterpart when commercial standards were not available. Cer(d18∶1/26∶1) ∼Cer(d18∶1/24∶1) and Cer(d18∶1/26∶0) ∼ Cer(d18∶1/24∶0).</p