Cleavage of RatA, <i>txpA</i>, and the RatA/<i>txpA</i> hybrid by RNase III and RNase Y.

Abstract

<p>(A) Cleavage of 5′ labeled <i>txpA</i> (0.5 pmol) alone or hybridized to a two-fold excess of unlabeled RatA by RNase III (3 ng) and RNase Y (2 µg) for the times indicated. A size standard is shown in lane M and an RNase T1 digestion of 5′ labeled <i>txpA</i> is shown in the lane labeled T1. (B) Cleavage of 5′ labeled RatA alone or hybridized to a two-fold excess of unlabeled <i>txpA</i> by RNase III and RNase Y for the times indicated. A size standard is shown in lane M and an RNase T1 digestion of 5′ labeled RatA is shown in the lane labeled T1.</p