Suppressors of the <i>rnc::spc</i> mutation lack the Skin prophage.

Abstract

<p>(A) Agarose gel showing multiplex PCR analysis of <i>rnc::spc</i> suppressors in wild-type (WT) and ΔSPβ strains. A PCR product corresponding to the reconstituted <i>ypqP</i> and <i>sigK</i> genes is indicative of excision of the SPβ and Skin prophages, respectively. Spontaneous Spc^R colonies show a PCR product for the <i>rnc</i> gene, while successfully deleted <i>rnc</i> strains do not give a corresponding PCR product. A DNA marker (bp) is shown in the lane labeled M. (B) Sequence comparison of the <i>txpA</i> gene in wild-type (wt) and the sup5 mutant shown in panel A.</p