The <i>rnc</i> gene can be deleted in strains lacking Skin and SPβ prophages.

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    Abstract

    <p>(A) Agar plates showing colony growth after transformation of wild-type (WT) strains and strains lacking up to three prophages with 5 µg CCB302 (<i>rnc::spc amyE::Pxyl-rnc</i> Cm) chromosomal DNA, selected for spectinomycin (left panel) or chloramphenicol (right panel) resistance. (B) Histograms showing transformation efficiencies (number of Spc<sup>R</sup> colonies/number Cm<sup>R</sup> colonies) for the different prophage deficient strains.</p

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      Last time updated on 16/03/2018