The <i>yonT</i> mRNA is stabilized in strains depleted for RNase III.

Abstract

<p>(A) Chromosomal context of the <i>yonT/as-yonT</i> toxin/antitoxin cassette present in the SPβ prophage. (B) and (C) Northern blots performed on RNA isolated at times (min) after rifampicin addition in strains depleted for RNase III (CCB288), probed for <i>yonT</i> and as-yonT, respectively. The <i>yonT</i> probe was a uniformly ³²P-labeled riboprobe, synthesized using a PCR fragment containing a T7 promoter as a template (oligos CC1101/1102; <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003181#pgen.1003181.s013" target="_blank">Table S2</a>). The blots were re-probed for 5S rRNA (5S) for normalization. Half-lives are given below each panel.</p