Expression and conformation analysis of HCV E2 glycoprotein in cell lysates.

Abstract

<p>(A) Expression analysis of HC-J6CH E2 protein by anti-E2 Western blot (B) E2 conformation analysis by E2 immunoprecipitation, as well as subsequent E2 detection by Western blot. β-actin detection was used as a loading control, while cell lysates from cells electroporated with JFH1 subgenomic replicons (SGR) were used as a negative control in both Western blot and immunoprecipitation experiments.</p