Buoyant density infectivity and RNA of WT and mutant viruses.
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Abstract
<p>The indicated viruses were resolved using an iodixanol step gradient. For each fraction HCV infectivity in Huh-7.5 cells and RNA (RT-qPCR) were determined. Values are plotted against the density of the respective fraction. The infectivity is expressed as a percentage of the total infectivity obtained from all fractions. All points represent the mean of duplicate infections measured in duplicate (n = 4, ± SD). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052651#s3" target="_blank">Results</a> are drawn from a representative experiment of two independent experiments.</p