Gene expression in PPDB-stimulated PBMC from BCG vaccinated and control cattle.

Abstract

<p>A. Protective efficacy after <i>M. bovis</i> challenge determined by pathology scoring. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003077#s2" target="_blank">Results</a> are expressed as total pathology scores <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003077#ppat.1003077-Vordermeier1" target="_blank">[5]</a>. Filled circles, unvaccinated control calves; open triangles, BCG vaccinated calves. (B, C). Transcription of the genes expressing IFN-γ (B) and IL-22 (C) following <i>in vitro</i> stimulation. PBMC were collected from BCG vaccinated (filled symbols) and controls (open symbols) before challenge (week -1) and after challenge with <i>M. bovis</i> at weeks 2, 4 and 8, and stimulated with PPD-B for 24 hours. cDNA was prepared and gene expression determined by RT-qPCR. Data are expressed as log10 relative expression levels compared to non-stimulated cells. Statistical analysis: 2-way ANOVA with Bonferroni post test, * P<0.05.</p

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