Polystyrene microtiter plate biofilm assay.

Abstract

<p>(A) Biofilm formation of the S8, S8<i>ΔclpP</i> and S8HB strains in the wells of 96-well polystyrene microtiter plates. The plates were stained with crystal violet. (B)The quantitative determination of biofilm formation. The S8 (▪), S8<i>ΔclpP</i> (•) and S8HB (⋄) strains were grown in BHI supplemented with NAD. The optical density of the bacterial biofilm formation was monitored by OD<sub>600</sub> after 12, 18, 24, 30, 36 and 42 h of incubation. Points indicate the mean values, and error bars indicate standard deviations.</p

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