B cells from cKS patients show increased resistance to spontaneous apoptosis and unaffected <i>in vivo</i> turnover.
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Abstract
<p>Apoptotic cells were detected by annexin V binding after 24 h culture in unstimulated conditions. <i>A</i>, representative flow cytometric analysis showing annexin V binding gated on total B cells, CD27<sup>−</sup>, MZ-like (CD27<sup>+</sup>IgD<sup>lo</sup>) and switched memory (CD27<sup>+</sup>IgD<sup>−</sup>) B cells, as indicated; comparison between one HC (upper row) and one cKS patient (lower row). B, B cells from cKS patients (grey bars) showed significantly lower annexinV binding than B cells from HCs (white bars); this lower annexin V binding was evident on CD27<sup>−</sup> and MZ-like (CD27<sup>+</sup>IgD<sup>lo</sup>) B cells, roughly composing the preimmune/natural effector compartment, but not on antigen-experienced switched memory (CD27<sup>+</sup>IgD<sup>−</sup>) B cells. Data shown as mean ± SE. <i>C, In vivo</i> cell turnover was estimated by Ki67 staining of freshly isolated PBMCs. Representative flow cytofluorimetric analysis showing Ki67 binding gated on total B cells, CD27<sup>−</sup> and CD27<sup>+</sup> B cells, as indicated; comparison between one HC (upper row) and one cKS patient (lower row). <i>D</i>, The proportion of Ki67<sup>+</sup> cells within total B cells, CD27<sup>−</sup> and CD27<sup>+</sup> B cells did not differ between cKS patients (grey bars) and HCs (white bars). Data shown as mean ± SE. <i>P</i>-values calculated using the Student <i>t</i> test for independent samples.*<i>P</i><.05; **<i>P</i><.01.</p