Read-through activation in Neo-Luc cell lines.

Abstract

<p>Total RNA prepared from individual cell lines isolated after electroporation with 1 µg of DNA was assayed by hybridization and protection from nuclease S1 digestion. Expression of <i>E1a-neo,</i> read-through transcription (RT), and <i>E1b-luc</i> produced the specific protected bands indicated in the diagram below the autoradiographic data. The diagram is laid out as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015704#pone-0015704-g004" target="_blank">Fig. 4</a>. Each lane represents a different cell line. He: Hela cells; DPM: cell lines derived from pNeoDPME1bLuc; GGT: cell lines derived from pNeoGGTE1bLuc; M: size standards.</p