Time course and extent of intracellular ATP release. A. Time course of air-stimulated ATP release.
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Abstract
<p>Extracellular ATP levels were quantified following a 15 second air exposure (Air) or an exchange of half the media (Control) in proliferating (Prolif) and calcium differentiated (Diff) NHEK cultures. Air-stimulated ATP release was significantly higher in differentiated cultures at the five minute time point, but remained significantly elevated for 25 minutes in both cultures. Values represent the mean +/β SEM for 13 air-exposed and 2 control dishes per group shown as % baseline. * <i>p</i><0.05 vs. 0 minute Prolif, + <i>p</i><0.05 vs. 0 minute Diff, # <i>p</i><0.05 Diff vs. Prolif. <b>B. Intracellular ATP following air exposure.</b> To determine the extent of intracellular ATP reduction after air exposure, levels of ATP in cell lysates of air-exposed and control confluent NHEK cultures were measured. Air-exposed cultures showed a significant reduction in intracellular ATP at the time of peak ATP release (7 minutes after exposure). This reduction was no longer significant by 60 minutes after air exposure. Values represent averages +/β SEM of 6 dishes per group, * <i>p</i><0.05 vs. control 7 minutes.</p