Influence of gonadectomy on the size of ALA in the mouse model for the disease.

Abstract

<p>Eight weeks old male and female mice were gonadectomized and intrahepatically challenged with 1×10⁵<i>E. histolytica</i> trophozoites seven weeks after castration. The animals were sacrificed 7 days post infection, the sizes of abscesses were determined and transformed into score values (score 0: no abscess; score 1: abscess <1 mm; score 2∶1 to 5 mm; score 3: >5 mm) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055694#pone-0055694-g001" target="_blank">Fig. 1A</a>) or the re-isolation rate of live <i>E. histolytica</i> trophozoites from abscessed liver tissue was determined (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055694#pone-0055694-g001" target="_blank">Fig. 1B</a>). Results were obtained from 3 independent trials each comprising 7 animals (statistics: Mann Whitney U test).</p