FoxQ1 suppress the NRXN3 expression in human glioma cell lines.

Abstract

<p><b>A</b>, Determination of FoxQ1 and NRXN3 expression in human glioma cell lines and normal human astrocytes using RT-qPCR (lower) and Western blot (upper). <b>B</b>, Up-regulation of NRXN3 mRNA and protein expression by overexpressing FoxQ1. FoxQ1 and NRXN3 expression levels in parental, control, SW1088-FoxQ1 cells by RT-qPCR (lower) and Western blot (upper). <b>C</b>, Down-regulation of NRXN3 mRNA and protein expression by depletion of FoxQ1 expression. FoxQ1 and NRXN3 expression in parental, control, and U-87MG-RNAi cells by RT-qPCR (lower) and Western blot (upper). <b>E+F</b>, Effect of FoxQ1 on NRXN3 promoter activity. Repression of the NRXN3 promoter in SW1088-FoxQ1 cells (E) and transactivation of the NRXN3 promoter in U-87MG-RNAi cells (F). Inhibition was calculated as a percentage relative to U-87MG cells and activation was calculated relative to SW1088 cells. Three independent experiments were conducted.</p