The NRXN3 as a transcriptional target of FoxQ1.

Abstract

<p><b>A</b>, Sequence and position of putative FoxQ1 binding sites on the NRXN3 promoter. <b>B</b>, ChIP assays were done with U-87MG cells. Chromatin fragments of the cells were immunoprecipitated with anti-FoxQ1 antibody or negative control IgG (middle) and subjected to PCR. We subjected 1% of the total cell lysates to PCR before immunoprecipitation as inputs. <b>C</b>, schematic structure of the NRXN3 promoter. The sequence of the FoxQ1 binding sites are shown in both wild-type (WT) and mutant (Mut) forms. <b>D+E</b>, Luciferase activity with or without mutations in NRXN3 promoter. U-87MG cells were transfected with the wild-type NRXN3 promoter or its mutants (D). SW1088 cells were co-transfected with the wild-type NRXN3 promoter or its mutants and pcDNA3.1-FoxQ1 (E). Luciferase activities were then determined. Three independent experiments were conducted. * <i>P</i><0.05</p